PELARGONIUM GRAVEOLENS (L'HÉR) ESSENTIAL OIL, ANTIOXIDANTS, ANTICYANOBACTERIAL AND ANTIALGAL COMPOUNDS: A SOLAR-BASED EXTRACTION TECHNIQUE AND YIELD PREDICTION USING LINEAR REGRESSION

kamal ezzarrouqy

doi:10.5937/ffr0-63027

kamal ezzarrouqy National Center for Studies and Research on Water and Energy (CNEREE), Cadi Ayyad University, Marrakech, Morocco

DOI: https://doi.org/10.5937/ffr0-63027

Keywords: geranium, solar hydro-destillation, essential oil, total phenolics/flavonoids, antioxidant activity, Microcystis aeruginosa, Chlorella

Abstract

Solar hydro-distillation (SHD) is presented as a new green technique to effectively extract the various phytochemicals from food by-products. To better understand the procedure, results, and advantages of such a green and sustainable source, the goal of this study was to compare the efficiency of SHD to extract essential oils from Pelargonium graveolens (L'Hér) while simultaneously releasing antioxidant compounds like polyphenols and flavonoids in the remaining phase of the solar still. The yields of the essential oils were 0.64 % and 0.60 % for SHD and conventional hydro-distillation (CHD), respectively. By using GC-MS analysis, 52 volatile components were identified. Citronellol (27.54 %–26.51 %), citronellyl formate (13.63 %–11.33 %), geraniol (11.94 %–10.97 %) and geranyl formate (8.31 %–5.84 %) represented the main components for SHD and CHD oils, respectively. For the extracts produced by SHD and maceration, total phenolic compounds (TPC), total flavonoid compounds (TFC), and antioxidant activity (AA) based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity were evaluated. The results showed that the SHD extract produced an extremely good extraction yield in TPC, TFC, and IC₅₀ (249.72 mg EAG.g^-1DM, 205.88 mg EQ.g^-1 DM, and IC₅₀=6.54 μg.mL^-1, respectively). Moreover, HPLC-UV analyses showed conservation in the extract after the SHD process of some identified compounds such as tyrosol (31.71 mg.g^-1 DM), gallic acid (24.67 mg.g^-1 DM), protocatechuic acid (22.59 mg.g^-1 DM), and ferulic acid (21.04 mg.g^-1 DM). On the other hand, the essential oil and the extract prepared by SHD showed an important anti-cyanobacterial/anti-algal activity against the bacterial/algal strain tested Microcystis aeruginosa and Chlorella sp., with growth inhibition diameters of 15.43 ± 0.32 mm, 16.73 ± 0.40 mm and 17.13 ± 0.35 mm, 25.96 ± 0.15 mm, respectively for essential oil and extract. A positive linear correlation was observed between antioxidants, polyphenols, flavonoids and anti-cyanobacterial/anti-algal activity for solar extracts. Results showed that SHD is a good alternative for recovering bioactive compounds from the Pelargonium graveolens (L'Hér.) with potent antioxidant, and anti-cyanobacterial/anti-algal activity.

References

Afzal, A., Munir, A., Ghafoor, A., & Alvarado, J. L. (2017). Development of hybrid solar distillation system for essential oil extraction. Renewable Energy, 113, 22–29. https://doi.org/10.1016/j.renene.2017.05.027

Al-Mijalli, S. H., Mrabti, H. N., Assaggaf, H., Attar, A. A., Hamed, M., Baaboua, A. E. L., Omari, N., El Menyiy, N., El Hazzoumi, Z., Sheikh, R. A., Zengin, G., Sut, S., Dall’Acqua, S., & Bouyahya, A., (2022). Chemical profiling and biological activities of pelargonium graveolens essential oils at three different phenological stages. Plants, 11(17), 1–16. https://doi.org/10.3390/plants11172226

Al-Saffar, A. Z., Al-Shanon, A. F., Al-Brazanc, S. L., Sabry, F. A., Hassan, F., & Hadi, N. A. (2016). Phytochemical analysis, antioxidant and cytotoxic potentials of pelargonium graveolens extract in human breast adenocarcinoma (MCF-7) Cell Line. Asian Journal of Biochemistry, 12(1), 16–26. https://doi.org/10.3923/ajb.2017.16.26

Ali, E. F., Hassan, F. A. S., & Elgimabi, M. (2018). Improving the growth, yield and volatile oil content of Pelargonium graveolens L. Herit by foliar application with moringa leaf extract through motivating physiological and biochemical parameters. South African Journal of Botany, 119, 383–389. https://doi.org/10.1016/j.sajb.2018.10.003

Atailia, I., & Djahoudi, A. (2015). Composition chimique et activité antibactérienne de l’huile essentielle de géranium rosat (Pelargonium graveolens L’Hér.) cultivé en Algérie. Phytotherapie, 13(3), 156–162. https://doi.org/10.1007/s10298-015-0950-2

Baldin, E. L. L., Aguiar, G. P., Fanela, T. L. M., Soares, M. C. E., Groppo, M., & Crotti, A. E. M. (2015). Bioactivity of Pelargonium graveolens essential oil and related monoterpenoids against sweet potato whitefly, Bemisia tabaci biotype B. Journal of Pest Science, 88(1), 191–199. https://doi.org/10.1007/s10340-014-0580-8

Ben ElHadj Ali, I., Tajini, F., Boulila, A., Jebri, M. A., Boussaid, M., Messaoud, C., & Sebaï, H. (2020). Bioactive compounds from Tunisian Pelargonium graveolens (L’Hér.) essential oils and extracts: α-amylase and acethylcholinesterase inhibitory and antioxidant, antibacterial and phytotoxic activities. Industrial Crops and Products, 158(May), 112951. https://doi.org/10.1016/j.indcrop.2020.112951

Boukhatem, M. N., Kameli, A., & Saidi, F. (2013). Essential oil of Algerian rose-scented geranium (Pelargonium graveolens): Chemical composition and antimicrobial activity against food spoilage pathogens. Food Control, 34(1), 208–213. https://doi.org/10.1016/j.foodcont.2013.03.045

Boukhris, M., Bouaziz, M., Feki, I., Jemai, H., El Feki, A., & Sayadi, S. (2012). Hypoglycemic and antioxidant effects of leaf essential oil of Pelargonium graveolens L’Hér. in alloxan induced diabetic rats. Lipids in Health and Disease, 11, 1–10. https://doi.org/10.1186/1476-511X-11-81

Boukhris, M., Hadrich, F., Chtourou, H., Dhouib, A., Bouaziz, M., & Sayadi, S. (2015). Chemical composition, biological activities and DNA damage protective effect of Pelargonium graveolens L’Hér. essential oils at different phenological stages. Industrial Crops and Products, 74, 600–606. https://doi.org/10.1016/j.indcrop.2015.05.051.

Box, J. D. (1983). Investigation of the Folin-Ciocalteau phenol reagent for the determination of polyphenolic substances in natural waters. Water Research, 17(5), 511–525. https://doi.org/10.1016/0043-1354(83)90111-2

Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28(1), 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5

Ćavar, S., & Maksimović, M. (2012). Antioxidant activity of essential oil and aqueous extract of Pelargonium graveolens L’Her. Food Control, 23(1), 263–267. https://doi.org/10.1016/j.foodcont.2011.07.031

Class, A. (2009). Determination of biophenols in olive oils by HPLC. International Olive Council, 29, 1–8. http://www.internationaloliveoil.org

Da Rosa, G. S., Vanga, S. K., Gariepy, Y., & Raghavan, V. (2019). Comparison of microwave, ultrasonic and conventional techniques for extraction of bioactive compounds from olive leaves (Olea europaea L.). Innovative Food Science and Emerging Technologies, 58, 102234. https://doi.org/10.1016/j.ifset.2019.102234.

Dewanto, V., Xianzhong, W., Adom, K. K., & Liu, R. H. (2002). Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. Journal of Agricultural and Food Chemistry, 50(10), 3010–3014. https://doi.org/10.1021/jf0115589

El Aanachi, S., Gali, L., Nacer, S. N., Bensouici, C., Dari, K., & Aassila, H. (2020). Phenolic contents and in vitro investigation of the antioxidant, enzyme inhibitory, photoprotective, and antimicrobial effects of the organic extracts of Pelargonium graveolens growing in Morocco. Biocatalysis and Agricultural Biotechnology, 29(June), 101819. https://doi.org/10.1016/j.bcab.2020.101819.

El Amrani Zerrifi, S., Tazart, Z., El Khalloufi, F., Oudra, B., Campos, A., & Vasconcelos, V. (2019). Potential control of toxic cyanobacteria blooms with Moroccan seaweed extracts. Environmental Science and Pollution Research, 26(15), 15218–15228. https://doi.org/10.1007/s11356-019-04921-9

Ezzarrouqy, K., Hejjaj, A., Idlimam, A., Ait, F., & Laila, N. (2022). Study of the energetic, exergetic, and thermal balances of a solar distillation unit in comparison with a conventional system during the distillation of rosemary leaves. Environmental Science and Pollution Research, 29(17), 25709-25722. https://doi.org/10.1007/s11356-021-17612-1

Ferraz, C. A., Pastorinho, M. R., Palmeira-de-Oliveira, A., & Sousa, A. C. A. (2022). Ecotoxicity of plant extracts and essential oils: A review. Environmental Pollution, 292(PB), 118319. https://doi.org/10.1016/j.envpol.2021.118319

Gomes, P. B., Mata, V. G., & Rodrigues, A. E. (2004). Characterization of Portuguese-grown geranium oil (Pelargonium sp.). Journal of Essential Oil Research, 16(5), 490–495. https://doi.org/10.1080/10412905.2004.9698779

Guinda, Á., Castellano, J. M., Santos-Lozano, J. M., Delgado-Hervás, T., Gutiérrez-Adánez, P., & Rada, M. (2015). Determination of major bioactive compounds from olive leaf. LWT - Food Science and Technology, 64(1), 431–438. https://doi.org/10.1016/j.lwt.2015.05.001

Hilali, S., Fabiano-Tixier, A. S., Elmaataoui, M., Petitcolas, E., Hejjaj, A., Aitnouh, F., Idlimam, A., Jacotet-Navarro, M., Bily, A., Mandi, L., & Chemat, F. (2018). Deodorization by solar steam distillation of rosemary leaves prior to solvent extraction of rosmarinic, carnosic, and ursolic acids. ACS Sustainable Chemistry and Engineering, 6(8), 10969–10979. https://doi.org/10.1021/acssuschemeng.8b02347

Hilali, S., Fabiano-Tixier, A. S., Ruiz, K., Hejjaj, A., Ait Nouh, F., Idlimam, A., Bily, A., Mandi, L., Chemat, F. (2019). Green extraction of essential oils, polyphenols, and pectins from orange peel employing solar energy: toward a zero-waste biorefinery. ACS Sustainable Chemistry and Engineering, 7(13), 11815-11822. https://doi.org/10.1021/acssuschemeng.9b02281

Jayasimha, B. (2006). Application of Scheffler reflectors for process industry. In Proceedings of the International Solar Cooker Conference, 8, (pp. 1–2). Pune, India. http://www.solare-bruecke.org/infoartikel/Papers_from_SCI_Conference_2006/Jayasimha_Rathod.pdf

Juliani, H. R., Koroch, A., Simon, J. E., Hitimana, N., Daka, A., Ranarivelo, L., & Langenhoven, P., (2006). Quality of geranium oils (Pelargonium species): Case studies in Southern and Eastern Africa. Journal of Essential Oil Research, 18, 116–121. https://doi.org/10.1080/10412905.2006.12067131

M’hamdi, Z., Bouymajane, A., Riffi, O., Rhazi Filali, F., Ettarchouch, M., ELhourri, M., & Amechrouq, A. (2024). Chemical composition and antibacterial activity of essential oil of Pelargonium graveolens and its fractions. Arabian Journal of Chemistry, 17(1), 105375. https://doi.org/10.1016/j.arabjc.2023.105375

Machalova, Z., Sajfrtova, M., Pavela, R., & Topiar, M. (2015). Extraction of botanical pesticides from Pelargonium graveolens using supercritical carbon dioxide. Industrial Crops and Products, 67, 310–317. https://doi.org/10.1016/j.indcrop.2015.01.070

Parejo, I., Viladomat, F., Bastida, J., Rosas-Romero, A., Flerlage, N., Burillo, J., & Codina, C. (2002). Comparison between the radical scavenging activity and antioxidant activity of six distilled and nondistilled Mediterranean herbs and aromatic plants. Journal of Agricultural and Food Chemistry, 50(23), 6882–6890. https://doi.org/10.1021/jf020540a

Ponomareva, E. I., & Molohova, E. I. (2017). Evaluation of the efficiency of supercritical carbon dioxide extraction for Pelargonium graveolens L’Her essential oil production. Russian Journal of Physical Chemistry B, 11(8), 1270–1275. https://doi.org/10.1134/S1990793117080097

Rana, V. S., Puram, K., Adhoiwala, L., & Dun, D. (2003). Chemical composition of the volatile oil of Ageratum conyzoides aerial parts. International Journal of Aromatherapy, 13(4), 203–206. https://doi.org/10.1016/S0962-4562(03)00080-8

Rao, B. R. R. (2002). Biomass yield, essential oil yield and essential oil composition of rose-scented geranium (Pelargonium species) as influenced by row spacings and intercropping with cornmint (Mentha arvensis L.f. piperascens Malinv. ex Holmes). Industrial Crops and Products, 16(2), 133–144. https://doi.org/10.1016/S0926-6690(02)00038-9

Rathore, S., Mukhia, S., Kumar, R., & Kumar, R. (2023). Essential oil composition and antimicrobial potential of aromatic plants grown in the mid-hill conditions of the Western Himalayas. Scientific Reports, 13(1), 1–13. https://doi.org/10.1038/s41598-023-31875-3

Riahi, L., Cherif, H., Miladi, S., Neifar, M., Bejaoui, B., Chouchane, H., Masmoudi, A. S., & Cherif, A. (2020). Use of plant growth promoting bacteria as an efficient biotechnological tool to enhance the biomass and secondary metabolites production of the industrial crop Pelargonium graveolens L’Hér. under semi-controlled conditions. Industrial Crops and Products, 154(June), 112721. https://doi.org/10.1016/j.indcrop.2020.112721

Scheffler, W. (2006). Introduction to the revolutionary design of Scheffler refectors. In Proceedings of the International Solar Cooker Conference. Aislingen, Germany.

Sompaga, S., Jyothi, B., Chekuri, S., Baburao, N., & Anupalli, R. (2016). Organic extracts of Pelargonium graveolens: phenol content, anti-oxidant and anti-bacterial activities. European Journal of Medicinal Plants, 17(1), 1–8. https://doi.org/10.9734/ejmp/2016/29040.

Souilem, S., Fki, I., Kobayashi, I., Khalid, N., Neves, M. A., Isoda, H., Sayadi, S., & Nakajima, M. (2017). Emerging technologies for recovery of value-added components from olive leaves and their applications in food/feed industries. Food and Bioprocess Technology, 10(2), 229–248. https://doi.org/10.1007/s11947-016-1834-7.

Szutt, A., Dołhańczuk-Sródka, A., & Sporek, M., (2019). Evaluation of chemical composition of essential oils derived from different Pelargonium species leaves. Ecological Chemistry and Engineering S, 26(4), 807–816. https://doi.org/10.1515/eces-2019-0057.

Tazart, Z., Manganelli, M., Scardala, S., Buratti, F. M., Di Gregorio, F. N., Douma, M., Mouhri, K., Testai, E., & Loudiki, M., (2021). Remediation strategies to control toxic cyanobacterial blooms: Effects of macrophyte aqueous extracts on microcystis aeruginosa (growth, toxin production and oxidative stress response) and on bacterial ectoenzymatic activities. Microorganisms, 9(8), 1782. https://doi.org/10.3390/microorganisms9081782.