Uporedno spektrofotometrijsko ispitivanje ukupnog sadržaja fenola i antioksidativnog kapaciteta merenih DPPH i ABTS metodama u zelenom povrću

  • Sofija Doganjić Univerzitet u Beogradu - Medicinski fakultet, Beograd, Srbija
  • Snežana Uskoković-Marković Univerzitet u Beogradu - Farmaceutski fakultet, Katedra za analitičku hemiju, Beograd, Srbija
  • Jelena Kukić Marković Univerzitet u Beogradu - Farmaceutski fakultet, Katedra za farmakognoziju, Beograd, Srbija
  • Leposava Pavun Univerzitet u Beogradu - Farmaceutski fakultet, Katedra za fizičku hemiju i instrumentalne metode, Beograd, Srbija
  • Aleksandra Janošević Ležaić Univerzitet u Beogradu - Farmaceutski fakultet, Katedra za fizičku hemiju i instrumentalne metode, Beograd, Srbija
DOI: https://doi.org/10.5937/arhfarm75-59496
Ključne reči: zeleno povrće, antioksidativna aktivnost, ukupan sadržaj fenola (TPC), DPPH, ABTS

Sažetak


Savremeni trendovi u ishrani podrazumevaju svakodnevni unos svežeg voća i povrća, uz konstataciju da su oni važni izvori prirodnih antioksidanata koji štite organizam od oksidativnog stresa i mogu doprineti prevenciji mnogih hroničnih bolesti. U ovim preporukama posebno se ističe zeleno povrće sa visokim ukupnim sadržajem fenola (TPC). Međutim, mora se postaviti pitanje da li je visok ukupan sadržaj fenola u zelenom povrću, kao što su prokelj, kupus, brokoli, peršun, kelj i spanać, pouzdan pokazatelj antioksidativne aktivnosti za regulisanje slobodnih radikala u organizmu. Cilj ovog rada je spektrofotometrijsko određivanje TPC i utvrđivanje korelacije između TPC i antioksidativnog potencijala metanol-vodenih ekstrakata odabranog zelenog povrća. U tu svrhu sa ekstraktima povrća urađeni su DPPH i ABTS testovi uklanjanja radikala. Dobijeni rezultati ukazuju na to da prokelj i peršun pokazuju najveću antioksidativnu aktivnost, dok ekstrakt kupusa pokazuje niže vrednosti. Pirsonova korelacija koja je korišćena za testiranje korelacije između TPC dobijene za ekstrakte zelenog povrća i rezultata DPPH i ABTS testova pokazuje umerenu korelaciju između vrednosti TPC i ABTS, dok je korelacija za TPC i DPPH statistički značajna.

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Objavljeno
2025/12/25
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Vol. 75 Br. Notebook 6 (2025): Arhiv za farmaciju
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