ANTIOKSIDATIVNI, ANTIMIKROBNI I SPAZMOLITIČKI EFEKTI HIDROETANOLNIH EKSTRAKATA MUSKATNE ŽALFIJE (SALVIA SCLAREA L.) PRIPREMLJENI METODOM DIGESTIJE
Sažetak
Muskatna žalfija (Salvia sclarea L.) poznata je u tradicionalnoj medicini kao aromatična i lekovita biljka koja se koristi u tretmanu respiratornih, digestivnih i menstrualnih tegoba i različitih inflamatornih procesa poput gingivitisa, stomatitisa, afti i artritisa. Cilj ovog rada bio je da ispita antioksidativne, antimikrobne i spazmolitičke efekte vodeno-etanolnih ekstrakata nadzemnih delova biljke S. sclarea, koji su sakupljeni u Malči, u okolini Niša u Srbiji. Pripremljena su dva ekstrakta tehnikom digestije sa etanolom od 80% i etanolom od 60% (HE80D i HE60D, redom). Ekstrakti su bili hemijski okarakterisani i tada je utvrđeno da sadrže značajne količine fenolnih jedinjenja, među kojima je ruzmarinska kiselina bila najdominantnija. Ispitivani ekstrakti su ispoljili značajne antioksidativne potencijale u sistemima 2,2-difenil-1-pikrilhidrazil i β-karoten/linolenska kiselina. Rezultati su pokazali da su ekstrakti naročito efikasni kao antilipoperoksidativni agensi. Ekstrakti su najizraženije antimikrobne efekte imali na bakteriju Staphyloccocus aureus, dok su oni koje su ispoljili prema bakterijama Bacillus cereus i Listeria monocytogenes bili umereni. Ekstrakti su pokazali značajnu inhibiciju spontanih kontrakcija glatkih mišića ileuma pacova. Pri maksimalnoj koncentraciji HE80D i HE60D ekstrakata od 1,5 mg/ml kontrakcije ileuma bile su smanjene za 40,45 ± 2,06% i 40,60 ± 1,22%, redom. Dalja istraživanja ekstrakata biljke S. sclarea treba usmeriti na detaljnija in vivo ispitivanja i kliničke studije kako bi se sagledala mogućnost njihove upotrebe u racionalnoj fitotetapiji.
Reference
Adzet T, Cainigueral S, Iglesias J. A chromatographic survey of polyphenols from Salvia species. Biochem System Ecol 1988; 16(1):29-32. [CrossRef]
Ahn K. The worldwide trend of using botanical drugs and strategies for developing global drugs. BMB Rep 2017; 50(3):111-6. [CrossRef] [PubMed]
Alimpić A. Mikromorfološke karakteristike Salvia amplexicaulis Lam., S. jurisicii Košanin i S. ringens Sibth. & Sm. (Lamiaceae), hemijski sastav i biološka aktivnost njihovih etarskih ulja i ekstrakata. Beograd: Biološki fakultet, Univerzitet u Beogradu; 2016.
Azwanida NN. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants 2015; 4(3):3-8. [CrossRef]
Bahmani M, Eftekhari Z, Jelodari M, Saki K, Abdollahi R, Majlesi M, et al. Effect of Iranian herbal medicines in dysmenorrhea phytotherapy. J Chem Pharm Res 2015; 7(2):519-26.
Bălăşoiu R, Biţă A, Stănciulescu EC, Rau G, Bejenaru C, Bejenaru LE, et al. UHPLC/MS assessment of polyphenolic acids and radical scavenging activity of Salvia species from the south-west of Romania flora. Farmacia 2023; 71(4):697-703. [CrossRef]
Baricevic D, Bartol T. The biological/pharmacological activity of the Salvia genus. In: Kintzios SE, editor. Sage: the genus Salvia. Amsterdam: Harwood Academic Publishers; 2000. p. 143.
Bone K, Mills SY. Principles and practice of phytotherapy, modern herbal medicine. Drugo izdanje. London: Churchill Livingstone Elsevier; 2013.
Caylak E. Determination of antioxidant and anti-inflammatory properties of Salvia aethiopis/sclarea and synthesized silver nanoparticles. Ann Med Res 2024; 31(2):169–76. [CrossRef]
Chew KK, Khoo MZ, Ng SY, Thoo YY, Aida WW, Ho CW. Effect of ethanol concentration, extraction time and extraction temperature on the recovery of phenolic compounds and antioxidant capacity of Orthosiphon stamineus extracts. Int Food Res J 2011; 18(4):1427-35.
Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing. 15th informational supplement, M100-S15. Wayne: Clinical and Laboratory Standards Institute; 2005.
Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999; 12(4):564-82. [CrossRef] [PubMed]
Dahchour A. Anxiolytic and antidepressive potentials of rosmarinic acid: A review with a focus on antioxidant and anti-inflammatory effects. Pharmacol Res 2022; 184:106421. [CrossRef] [PubMed]
Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 2010; 15(10):7313-52. [CrossRef] [PubMed]
Dweck AC. Folklore and cosmetic use of various Salvia species. In: Kintzios SE, editor. Sage: the genus Salvia. Amsterdam: Harwood Academic Publishers; 2000. p. 20-1. [CrossRef]
Eisenman SW, Zaurov DE, Struve L. Medicinal plants of Central Asia: Uzbekistan and Kyrgyzstan. Berlin: Springer Science & Business Media; 2012. [CrossRef]
El-Seedi HR, El-Said AM, Khalifa SA, Goransson U, Bohlin L, Borg-Karlson AK, Verpoorte R. Biosynthesis, natural sources, dietary intake, pharmacokinetic properties, and biological activities of hydroxycinnamic acids. J Agric Food Chem 2012; 60(44):10877–95. [CrossRef] [PubMed]
European Directorate for the Quality of Medicines & HealthCare. European Pharmacopoeia. 11th ed. Strasbourg, France: Council of Europe; 2023.
European Medicines Agency. EMA/HMPC/277152/2015. European Union herbal monograph on Salvia officinalis L., folium; Europe-an Medicines Agency: Amsterdam, The Netherlands, 2016.
FDA – Food and drug administration. Bad Bug Book. Foodborne pathogenic microorganisms and natural toxins handbook. Second edition. Silver Spring: Center for food safety and applied nutrition (CFSAN) of the Food and drug administration (FDA), U.S. Department of health and human services; 2012.
Formagio ASN, Volobuff CRF, Santiago M, Cardoso CAL, Vieira MC, Pereira ZV. Evaluation of antioxidant activity, total flavonoids, tannins and phenolic compounds in Psychotria leaf extracts. Antioxidants 2014; 3(4):745-57. [CrossRef] [PubMed]
Grasa L, Rebollar E, Arruebo MP, Plaza MA, Murillo MD. The role of Ca+2 in the contractility of rabbit small intestine in vitro. J Physiol Pharmacol 2004; 55(3):639-50. [PubMed]
Guarrera PM. Traditional phytotherapy in Central Italy (Marche, Abruzzo, and Latium). Fitoterapia 2005; 76(1):1-25. [CrossRef] [PubMed]
Hagerman A, Harvey-Mueller I, Makkar HPS. Quantification of tannins in tree foliage - a laboratory manual. Vienna; FAO/IAEA; 2000.
Jasicka-Misiak I, Poliwoda A, Petecka M, Buslovych O, Shlyapnikov VA, Wieczorek PP. Antioxidant phenolic compounds in Salvia officinalis L. and Salvia sclarea L. Ecol Chem Eng S 2018; 25(1):133-42. [CrossRef]
Joukar M, Larijani K, Farjam MH, Givianrad MH, Nematollahi F. Chemical and antimicrobial properties of a flavonoid extracted from the Cleome turkmena Bobrov. Journal of Chemical Reactivity and Synthesis 2022; 12(4):232-8.
Jovanović M. Praktikum iz farmaceutske tehnologije sa biofarmacijom I deo. Drugo izdanje. Beograd; Nijansa, Zemun: 2004.
Kan Y, Gökbulut A, Kartal M, Konuklugil B, Yılmaz G. Development and validation of a LC method for the analysis of phenolic acids in Turkish Salvia species. Chromatographia 2007; 66(1):S147-52. [CrossRef]
Karaki H, Weiss G. Calcium release in smooth muscles. Life Sci 1988; 42(2):111-22. [CrossRef] [PubMed]
Karaman S, Kocabas YZ. Traditional medicinal plants of K. Maras (Turkey). The Sciences 2001; 1(3):125-8. [CrossRef]
Khoury M, Stien D, Eparvier V, Ouaini N, El Beyrouthy M. Report on the medicinal use of eleven Lamiaceae species in Lebanon and rationalization of their antimicrobial potential by examination of the chemical composition and antimicrobial activity of their essential oils. Evid Based Complement Alternat Med 2016; 2016:2547169. [CrossRef] [PubMed]
Kitić D. Divlji bosiljak - hemijsko i mikrobiološko ispitivanje. Beograd: Zadužbina Andrejević; 2006.
Kitić N, Živković J, Šavikin K, Randjelović M, Jovanović M, Kitić D, et al. Spasmolytic activity of Gentiana lutea L. root extracts on the rat ileum: underlying mechanisms of action. Plants 2024; 13(3):453. [CrossRef] [PubMed]
Kostić M, Zlatković B, Miladinović B, Živanović S, Mihajilov‐Krstev T, Pavlović D, et al. Rosmarinic acid levels, phenolic contents, antioxidant and antimicrobial activities of the extracts from Salvia verbenaca L. obtained with different solvents and procedures. J Food Biochem 2015; 39(2):199-208. [CrossRef]
Kostić, M, Kitić D, Petrović MB, Jevtović-Stoimenov T, Jović M, Petrović A, et al. Anti-inflammatory effect of the Salvia sclarea L. ethanolic extract on lipopolysaccharide-induced periodontitis in rats. J Ethnopharmacol 2017; 199:52–9. [CrossRef] [PubMed]
Lawrence BM. Production of clary sage oil and sclareol in North America. 4emes Recontres Internacionales, Nyons. 5-7 Dec 1994. p. 41-58.
Lee KA, Moon SH, Kim KT, Mendonca AF, Paik HD. Antimicrobial effects of various flavonoids on Escherichia coli O157: H7 cell growth and lipopolysaccharide production. Food Sci Biotechnol 2010; 19(1):257-61. [CrossRef]
Lee KA, Moon SH, Kim KT, Mendonca AF, Paik HD. Antimicrobial effects of various flavonoids on Escherichia coli O157: H7 cell growth and lipopolysaccharide production. Food Sci Biotechnol 2010; 19(1):257-61. [CrossRef]
Leporatti ML, Pavesi A, Posocco E. Phytotherapy in the Valnerina Marche (Central Italy). J Ethnopharmacol 1985; 14(1):53-63. [CrossRef] [PubMed]
Lis-Balchin M, Hart S. A preliminary study of the effect of essential oils on skeletal and smooth muscle in vitro. J Ethnopharmacol 1997; 58(3):183-7. [CrossRef] [PubMed]
Lourenço SC, Moldão-Martins M, Alves VD. Antioxidants of Natural Plant Origins: From Sources to Food Industry Applications. Molecules 2019; 24(22):4132. [CrossRef] [PubMed]
Lu Y, Foo LY. Polyphenolics of Salvia—a review. Phytochemistry 2002; 59(2):117-40. [CrossRef] [PubMed]
Mervić M, Bival Štefan M, Kindl M, Blažeković B, Marijan M, Vladimir-Knežević S. Comparative antioxidant, anti-acetylcholinesterase and anti-α-glucosidase activities of mediterranean Salvia species. Plants 2022; 11(5):625. [CrossRef] [PubMed]
Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 2004; 26(2):211-9.
Nkengla A. Investigation of the in vitro bioavailability of luteolin from modified preparations of Artemisia afra. Magister thesis. Bellville, South Africa: University of the Western Cape; 2014.
Onder A, Izgi MN, Cinar AS, Zengin G, Yilmaz MA. The characterization of phenolic compounds via LC-ESI-MS/MS, antioxidant, enzyme inhibitory activities of Salvia absconditiflora, Salvia sclarea, and Salvia palaestina: A comparative analysis. S Afr J Bot 2022; 150:313-22. [CrossRef]
Ozcelik B, Kartal M, Orhan I. Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids. Pharm Biol 2011; 49(4):396-402. [CrossRef] [PubMed]
Parker D, Prince A. Immunopathogenesis of Staphylococcus aureus pulmonary infection. Semin Immunopathol 2012; 34:281-97. [CrossRef] [PubMed]
Parsaei P, Bahmani M, Naghdi N, Asadi-Samani M, Rafieian-Kopaei M, Tajeddini P, et al. Identification of medicinal plants effective on common cold: An ethnobotanical study of Shiraz, South Iran. Pharm Lett 2016; 8(2):90-7.
Paur I, Carlsen MH, Halvorsen BL, Blomhoff R. Antioxidants in herbs and spices. In: Benzie IFF, Wachtel-Galor S, editors. Herbal medicine: biomolecular and clinical aspects. 2nd edition. Boca Raton (FL): CRC Press/Taylor & Francis; 2011. [CrossRef] [PubMed]
Peana AT, Moretti M. Pharmacological activities and applications of Salvia sclarea L. and Salvia desoleana essential oils. In: Atta-ur-Rahman, editor. Studies in Natural Products Chemistry. Vol. 26. Amsterdam: Elsevier; 2002. [CrossRef]
Petersen M, Simmonds MJ. Rosmarinic acid. Phytochemistry 2003; 62(2):121-5. [CrossRef] [PubMed]
Quradha MM, Duru ME, Kucukaydin S, Tamfu AN, Iqbal M, Bibi H, Khan R, Ceylan O. Comparative assessment of phenolic composition profile and biological activities of green extract and conventional extracts of Salvia sclarea. Sci Rep 2024; 14(1):1885. [CrossRef] [PubMed]
Randjelović M, Branković S, Jovanović M, Kitić N, Živanović S, Mihajilov-Krstev T, et al. An in vitro and in silico characterization of Salvia sclarea L. methanolic extracts as spasmolytic agents. Pharmaceutics 2023; 15(5):1376. [CrossRef] [PubMed]
Randjelović M, Branković S, Miladinović B, Milutinović M, Živanović S, Mihajilov-Krstev T, et al The benefits of Salvia sclarea L. ethanolic extracts on gastrointestinal and respiratory spasms. S Afr J Bot 2022; 150:621-32. [CrossRef]
Rashed K, Ćirić A, Glamočlija J, Soković M. Antibacterial and antifungal activities of methanol extract and phenolic compounds from Diospyros virginiana L. Ind Crop Prod 2014; 59:210-5. [CrossRef] [PubMed]
Rauha JP, Remes S, Heinonen M, Hopia A, Kähkönen M, Kujala T, et al. Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. Int J Food Microbiol 2000; 56(1):3-12. [CrossRef] [PubMed]
Roginsky V, Lissi EA. Review of methods to determine chain-breaking antioxidant activity in food. Food Chem 2005; 92(2):235-54. [CrossRef]
Sartoratto A, Machado ALM, Delarmelina C, Figueira GM, Duarte MCT, Rehder VLG. Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil. Braz J Microbiol 2004; 35(4):275-80. [CrossRef]
Savić L. Metode ekstrakcije biljnih materijala: Uporedna analiza cirkulatorne ekstrakcije i ekstrakcije primenom superkritičnog ugljen-dioksida. Lek Sirov 2014; 34:93-103.
Scalbert A, Morand C, Manach C, Remesy C. Absorption and metabolism of polyphenols in the gut and impact on health. Biomed Pharmacother 2002; 56(6):276-82. [CrossRef] [PubMed]
Shaheen UY, Hussain MH, Ammar HA. Cytotoxicity and antioxidant activity of new biologically active constituents from Salvia lanigra and Salvia splendens. Pharmacognosy Journal 2011; 3(21):36-48. [CrossRef]
Sharopov F, Valiev A, Sobeh M, Arnold E, Winka M. Bioactivity of three Salvia species in relation to their total phenolic and flavonoid contents. Pharm Chem J 2018; 1-5. [CrossRef]
Sonboli A, Babakhani B, Mehrabian AR. Antimicrobial activity of six constituents of essential oil from Salvia. Z Naturforsch C 2006; 61(3-4):160-4. [CrossRef] [PubMed]
Sonboli A, Babakhani B, Mehrabian AR. Antimicrobial activity of six constituents of essential oil from Salvia. Z Naturforsch C 2006; 61(3-4):160-4. [CrossRef] [PubMed]
Spagnol CM, Assis RP, Brunetti IL, Isaac VL, Salgado HR, Corrêa MA. In vitro methods to determine the antioxidant activity of caffeic acid. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2019; 219:358-66. [CrossRef] [PubMed]
Sulaiman ISC, Basri M, Masoumi HRF, Chee WJ, Ashari SE, Ismail M. Effects of temperature, time, and solvent ratio on the extraction of phenolic compounds and the anti‑radical activity of Clinacanthus nutans Lindau leaves by response surface methodology. Chem Cent J 2017; 11(1):54. [CrossRef] [PubMed]
Tepe B, Eminagaoglu O, Akpulat HA, Aydin E. Antioxidant potentials and rosmarinic acid levels of the methanolic extracts of Salvia verticillata (L.) subsp. verticillata and S. verticillata (L.) subsp. amasiaca (Freyn & Bornm.) Bornm. Food Chem 2007; 100(3):985-9. [CrossRef]
Tian C, Liu X, Chang Y, Wang R, Lv T, Cui C, Liu M. Investigation of the anti-inflammatory and antioxidant activities of luteolin, kaempferol, apigenin and quercetin. S Afr J Bot 2021; 137:257-64. [CrossRef]
Todorov S, Philianos S, Petkov V, Harvala C, Zamfirova R, Olimpiou H. Experimental pharmacological study of three species from genus Salvia. Acta Physiol Pharmacol Bulg 1984; 10(2):13-20. [PubMed]
Ulubelen A, Topcu G, Eri C, Sonmez U, Kartal M, Kurucu S, et al. Terpenoids from Salvia sclarea. Phytochemistry 1994; 36(4):971-4. [CrossRef] [PubMed]
Vergine M, Nicolì F, Negro C, Luvisi A, Nutricati E, Accogli RA, et al. Phytochemical profiles and antioxidant activity of Salvia species from southern Italy. Rec Nat Prod 2019; 13(3):215. [CrossRef]
Wu YB, Ni ZY, Shi QW, Dong M, Kiyota H, Gu YC, et al. Constituents from Salvia species and their biological activities. Chem Rev 2012; 112(11):5967-6026. [CrossRef] [PubMed]
Yalcin H, Ozturk I, Tulukcu E, Sagdic O. Effect of γ‐irradiation on bioactivity, fatty acid compositions and volatile compounds of clary sage seed (Salvia sclarea L.). J Food Sci 2011; 76(7):C1056-61. [CrossRef] [PubMed]
Zengin G, Senkardes I, Mollica A, Picot-Allain CMN, Bulut G, Dogan A, et al. New insights into the in vitro biological effects, in silico docking and chemical profile of clary sage–Salvia sclarea L. Comp Biol Chemy 2018; 75:111-9. [CrossRef] [PubMed]
Zhussupova A, Zhumaliyeva G, Ogay V, Issabekova A, Ross SA, Zhusupova GE. Immunomodulatory effects of plant extracts from Salvia deserta Schang. and Salvia sclarea L. Plants 2022; 11(20):2690. [CrossRef] [PubMed]
