Synergistic effects of serum albumin and HDL cholesterol concentrations on serum oxidized LDL cholesterol concentration in obese individuals with normal glycoregulation and patients with type 2 diabetes mellitus

Radoslav Pejin; Andrijana Milankov; Tijana  Ičin; Ivana Bajkin; Jelena Niković; Aleksandar Jovanović; Ilija Tanackov

doi:10.2298/VSP240626038P

Radoslav Pejin University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia; University Clinical Center of Vojvodina, †Clinic for Endocrinology, Novi Sad, Serbia
Andrijana Milankov University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia; University Clinical Center of Vojvodina, Clinic for Endocrinology, Novi Sad, Serbia
Tijana Ičin University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia; University Clinical Center of Vojvodina, Clinic for Endocrinology, Novi Sad, Serbia
Ivana Bajkin University of Novi Sad, Faculty of Medicine, University Clinical Center of Vojvodina, Clinic for Endocrinology, Diabetes and Metabolic Disorders
Jelena Niković University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia; University Clinical Center of Vojvodina, Clinic for Neurology, Novi Sad, Serbia
Aleksandar Jovanović University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia; University Clinical Center of Vojvodina, Clinic for Neurology, Novi Sad, Serbia
Ilija Tanackov University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia; Faculty of Technical Sciences, Novi Sad, Serbia

DOI: https://doi.org/10.2298/VSP240626038P

Keywords: albumins;, diabetes mellitus, type 2;, lipoproteins, hdl;, lipoproteins, ldl;, obesity;, oxidative stress

Abstract

Background/Aim. Serum albumin and high-density lipoprotein (HDL) cholesterol molecules have multiple physiological functions, including an antioxidant role in neutralizing the harmful effects of oxidized low-density lipoprotein (oxLDL). In obese individuals, albumin and HDL cholesterol molecules are unable to counteract the unfavorable effects of oxLDL cholesterol adequately. The aim of the study was to examine the functional relationships between oxLDL cholesterol, HDL cholesterol, and serum albumin. Methods. The study included 30 obese individuals with newly diagnosed type 2 diabetes mellitus (before and after a three-month treatment with metformin), 30 obese individuals with normal glucose tolerance, and 30 normal-weight subjects (control group). The groups were age- and sex-matched. Results. Both qualitative and quantitative changes in the levels of HDL cholesterol and albumin were detected among the groups. Statistically significant changes were found in the linear correlations between albumin and oxLDL cholesterol among the study groups. Furthermore, by forming a synergistic influence of independent variables (HDL cholesterol and albumin), expressed through a complex polynomial of the dependent variable (oxLDL) of the quadratic type, statistically significant qualitative and quantitative changes in maximal oxLDL values were observed in all examined groups. Conclusion. The results of our study indicate a potential synergistic effect of albumin and HDL cholesterol in the prevention of oxidative damage, as well as a possible alteration in the quality of the ratio of these parameters in relation to oxLDL cholesterol molecules under conditions characterized by increased oxidative stress.

References

Belinskaia DA, Voronina PA, Shmurak VI, Jenkins RO, Goncharov NV. Serum albumin in health and disease: es-terase, antioxidant, transporting and signaling properties. Int J Mol Sci 2021; 22(19): 10318.

Feng J, Wang Y, Li W, Zhao Y, Liu Y, Yao X, et al. High levels of oxidized fatty acids in HDL impair the antioxidant func-tion of HDL in patients with diabetes. Front Endocrinol 2022; 13: 993193.

Brites F, Martin M, Guillas I, Kontush A. Antioxidative activity of high-density lipoprotein (HDL): Mechanistic insights into potential clinical benefit. BBA Clin 2017; 8: 66–77.

Chaudhary P, Janmeda P, Docea AO, Yeskaliyeva B, Abdul Razis AF, Modu B, et al. Oxidative stress, free radicals and antioxi-dants: potential crosstalk in the pathophysiology of human diseases. Front Chem 2023; 11: 1158198.

Sastre-Oliva T, Corbacho-Alonso N, Rodriguez-Sanchez E, Mercado-García E, Perales-Sanchez I, Hernandez-Fernandez G, et al. Al-bumin redox modifications promote cell calcification reflect-ing the impact of oxidative status on aortic valve disease and atherosclerosis. Antioxidants 2024; 13(1): 108.

Fogelman AM. When good cholesterol goes bad. Nat Med 2004; 10(9): 902–3.

Mahfouz MM, Kummerlow FA. High density lipoprotein can modulate the inhibitory effect of Ox-LDL on prostacyclin generation by rat aorta in vitro. Prostaglandins Other Lipid Mediat 2003; 72(3–4): 91–114.

Bowry VW, Stanley KK, Stocker R. High density lipoprotein is the major carrier of lipid hydroperoxides in human blood plasma from fasting donors. Proc Natl Acad Sci USA 1992; 89(21): 10316–20.

Hoekstra M, Van Eck M. High-density lipoproteins and non-alcoholic fatty liver disease. Atheroscler Plus 2023; 53: 33–41.

Poznyak AV, Sukhorukov VN, Surkova R, Orekhov NA, Orekhov AN. Glycation of LDL: AGEs, impact on lipoprotein func-tion, and involvement in atherosclerosis. Front Cardiovasc Med 2023; 10: 1094188.

Zoanni B, Brioschi M, Mallia A, Gianazza E, Eligini S, Carini M, et al. Novel insights about albumin in cardiovascular diseases: Focus on heart failure. Mass Spectrom Rev 2023; 42(4): 1113–28.

Piarulli F, Banfi C, Brioschi M, Altomare A, Ragazzi E, Cosma C, et al. The burden of impaired serum albumin antioxidant properties and glyco-oxidation in coronary heart disease pa-tients with and without type 2 Diabetes Mellitus. Antioxi-dants (Basel) 2022; 11(8): 1501.

Chadwick AC, Holme RL, Chen Y, Thomas MJ, Sorci-Thomas MG, Silverstein RL, et al. Acrolein impairs the cholesterol transport functions of high density lipoproteins. PLoS One 2015; 10(4): e0123138.

Ito F, Ito T. High-density lipoprotein (HDL) triglyceride and oxidized HDL: New lipid biomarkers of lipoprotein-related atherosclerotic cardiovascular disease. Antioxidants (Ba-sel) 2020; 9(5): 362.