The effect of the aqueous and methanol fennel stem extracts (Foeniculum vulgare Miller) on isolated rat ileum contractility

Marija  Gočmanac-Ignjatović; Dušanka Kitić; Mirjana Radenković; Milica Kostić; Milica Milutinović; Gorana  Nedin-Ranković; Suzana Branković

doi:10.2298/VSP161001391G

Marija Gočmanac-Ignjatović University of Niš, Faculty of Medicine, Department of Physiology, Niš, Serbia
Dušanka Kitić University of Niš, Faculty of Medicine, Department of Pharmacy, Faculty of Medicine, Niš, Serbia
Mirjana Radenković University of Niš, Faculty of Medicine, Department of Physiology, Niš, Serbia
Milica Kostić University of Niš, Faculty of Medicine, Department of Pharmacy, Niš, Serbia
Milica Milutinović University of Niš, Faculty of Medicine, Department of Pharmacy, Niš, Serbia
Gorana Nedin-Ranković University of Niš, Faculty of Medicine, Department of Pharmacology, Niš, Serbia
Suzana Branković University of Niš, Faculty of Medicine, Department of Physiology, Niš, Serbia

DOI: https://doi.org/10.2298/VSP161001391G

Keywords: ileum;, rats;, foeniculum;, acetylcholine;, potassium chloride;, treatment outcome.

Abstract

Background/Aim. The fennel (Foeniculum vulgare Miller, Apiaceae) has a long history of use as traditional herb medicine due to its carminative properties. The study was aimed to investigate the effects of aqueous and methanol fennel stem extracts on intestinal activity. Methods. Relaxant activity of aqueous and methanol fennel stem extracts was evaluated in vitro in three experimental models: spontaneous contraction, acetylcholine and potassium chloride (KCl)-induced contraction of an isolated rat ileum. The composition of aqueous and methanol fennel stem extracts was qualitatively analyzed using the high performance liquid chromatographic (HPLC) analysis. Results. In the presence of an aqueous fennel stem extract at a concentration of 3 mg/mL, the inhibition of the spontaneous contractions of isolated rat ileum was 35.05% ± 3.57%. In presence of a methanol fennel stem extract at the same concentration, the maximum reduction of the spontaneous contractions was 48.91% ± 6.31%. The extracts in a concentration-dependent manner significantly inhibited the acetylcholine and KCl induced contractions of the isolated rat ileum (p < 0.01). The following components were identified in fennel methanol stem extract: 3-caffeoylquinic acid, chlorogenic acid, 4-caffeoylquinic acid, 1,3-dicaffeoylquinic acid, rutin, miquelianin, quercetin heterosides, 1,5-dicaffeoylquinic acid, 1,4-dicaffeoylquinic acid, apigenin and rosmarinic acid. In an aqueous extract, their presence is found in trace amounts. Conclusion. The results of this study showed that the aqueous and methanol fennel stem extracts have spasmolytic effects on the intestinal smooth muscle and may be used for the control of intestinal motility.

Author Biographies

Marija Gočmanac-Ignjatović, University of Niš, Faculty of Medicine, Department of Physiology, Niš, Serbia

Fiziologija

Asistent

Dušanka Kitić, University of Niš, Faculty of Medicine, Department of Pharmacy, Faculty of Medicine, Niš, Serbia

Farmacija

Redovni profesor

Mirjana Radenković, University of Niš, Faculty of Medicine, Department of Physiology, Niš, Serbia

Fiziologija

Redovni profesor

Milica Kostić, University of Niš, Faculty of Medicine, Department of Pharmacy, Niš, Serbia

Farmacija

Asistent

Milica Milutinović, University of Niš, Faculty of Medicine, Department of Pharmacy, Niš, Serbia

Farmacija

Asistent

Gorana Nedin-Ranković, University of Niš, Faculty of Medicine, Department of Pharmacology, Niš, Serbia

Farmakologija sa toksikologijom

Asistent

Suzana Branković, University of Niš, Faculty of Medicine, Department of Physiology, Niš, Serbia

Fiziologija

Vanredni profesor

References

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