Influence of immortelle (Helichrysum italicum (Roth) G. Don) and lavender (Lavandula angustifolia Mill.) hydrolates on oxidative stress and antioxidative parameters in human serum

Nemanja Krgović; Gordana Miljak Vujičić; Jelena Kukić Marković; Milena Simić; Mijo Miljak; Jelena Kotur Stevuljević

doi:10.5937/arhfarm75-57851

Nemanja Krgović Institute for Medicinal Plants Research „Dr. Josif Pančić“, Belgrade, Serbia
Gordana Miljak Vujičić World of Plants – Limited liability company for flower production, trade and services, Vodnjan, Croatia
Jelena Kukić Marković University of Belgrade – Faculty of Pharmacy, Department of Pharmacognosy, Belgrade, Serbia
Milena Simić University of Belgrade - Faculty of Pharmacy, Department of Organic Chemistry, Belgrade, Serbia
Mijo Miljak World of Plants – Limited liability company for flower production, trade and services, Vodnjan, Croatia
Jelena Kotur Stevuljević University of Belgrade - Faculty of Pharmacy, Department of Medical Biochemistry, Belgrade, Serbia

DOI: https://doi.org/10.5937/arhfarm75-57851

Keywords: hydrolates, human serum, oxidative stress, antioxidant activity

Abstract

A number of studies suggest that different antioxidants play important roles in the body's antioxidant defense system. Hydrolates, by-products of essential oils, commonly obtained by steam distillation, are recognized to possess remarkable pharmacological activities, in particular an antioxidant activity. This study aimed to investigate and compare the antioxidant properties of immortelle hydrolate (IH) and lavender hydrolate (LH) using an ex vivo platform, i.e. human serum. To assess their impact on oxidative stress / antioxidative parameters, spectrophotometric biochemical assays were applied. The GC/MS analysis revealed that the main constituents were italidione I (17.2%), linalool (15.6%), a-terpineol (13.7%), terpinen-4-ol (6.3%) and nerol (5.2%) in IH, and linalool (19.6%), terpinen-4-ol (17.0%), a-terpineol (13.6%) and borneol (5.8%) in LH. The results showed that serum samples with LH, along with tert-butyl hydroperoxide as a pro-oxidant, had lower concentrations of the TOP (total oxidant potency) parameter, and slightly higher concentrations of the TAC (total antioxidant capacity) and SHG (total sulfhydryl groups) parameters compared to IH samples. Moreover, a significant difference in the OSI (oxidative stress index) parameter was observed (IH vs. LH – 49 (41–58) vs. 70 (61–75), p<0.05). The current ex vivo platform demonstrated IH and LH distinct antioxidant potency, highlighting LH as a potentially stronger antioxidant than IH.

References

Chatterjee S. Oxidative Stress, Inflammation, and Disease. In: Dziubla T, Butterfield DA, editors. Oxidative Stress and Biomaterials. Cambridge (MA): Academic Press; 2016; pp. 35–58.

Dobros N, Zawada KD, Paradowska K. Phytochemical Profiling, Antioxidant and Anti-Inflammatory Activity of Plants Belonging to the Lavandula Genus. Molecules. 2023;28(1). doi: 10.3390/molecules28010256.

Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, et al. Oxidative stress, aging, and diseases. Clin Interv Aging. 2018;13:757–72. doi: 10.2147/cia.s158513.

Davies KJA. Oxidative stress: the paradox of aerobic life. Biochem Soc Symp. 1995;61:1–31. doi: 10.1042/bss0610001.

Kasote DM, Katyare SS, Hegde MV, Bae H. Significance of Antioxidant Potential of Plants and its Relevance to Therapeutic Applications. Int J Biol Sci. 2015;11(8):982–91. doi: 10.7150/ijbs.12096.

Amorati R, Foti MC, Valgimigli L. Antioxidant Activity of Essential Oils. J Agric Food Chem. 2013;61(46):10835–47. doi: 10.1021/jf403496k.

Kar S, Gupta P, Gupta J. Essential Oils: Biological Activity Beyond Aromatherapy. Nat Prod Sci. 2018;24(3). doi: 10.20307/nps.2018.24.3.139.

Jakubczyk K, Tuchowska A, Janda-Milczarek K. Plant hydrolates – Antioxidant properties, chemical composition and potential applications. Biomed Pharmacother. 2021;142:112033. doi: 10.1016/j.biopha.2021.112033.

Aćimović MG, Tešević VV, Smiljanić KT, Cvetković MT, Stanković JM, Kiprovski BM, et al. Hydrolates: By-products of essential oil distillation: Chemical composition, biological activity and potential uses. Adv Technol. 2020;9:54–70. doi: 10.5937/savteh2002054A.

Shafie MH, Kamal ML, Abdul Razak NA, Hasan S, Uyup NH, Abdul Rashid NF, Zafarina Z. Antioxidant and Antimicrobial Activity of Plant Hydrosol and Its Potential Application in Cosmeceutical Products. Jundishapur J Nat Pharm Prod. 2022;17(4):e124018. doi: 10.5812/jjnpp-124018.

Hamedi A, Afifi M, Etemadfard H. Investigating chemical composition and indications of hydrosol soft drinks (aromatic waters) used in persian folk medicine for women’s hormonal and reproductive health conditions. J Evid Based Complement Altern Med. 2017;22:824–39.

Hamedi A, Pasdaran A, Zebarjad Z, Moein M. A survey on chemical constituents and indications of aromatic waters soft drinks (hydrosols) used in persian nutrition culture and folk medicine for neurological disorders and mental health. J Evid Based Complement Altern Med. 2017;22:744–52.

Ninčević T, Grdiša M, Šatović Z, Jug-Dujaković M. Helichrysum italicum (Roth) G. Don: Taxonomy, biological activity, biochemical and genetic diversity. Ind Crops Prod. 2019;138:111487. doi: 10.1016/j.indcrop.2019.111487.

Kokalj Ladan M, Kočevar Glavač N. GC–MS Analysis of A Helichrysum italicum Hydrosol: Sensitivity, Repeatability and Reliability of Solvent Extraction versus Direct Hydrosol Analysis. Appl Sci. 2022;12(19):10040. doi: 10.3390/app121910040.

Furlan V, Bren U. Helichrysum italicum: From Extraction, Distillation, and Encapsulation Techniques to Beneficial Health Effects. Foods. 2023;12(4). doi: 10.3390/foods12040802.

Kladar NV, Anačkov GT, Rat MM, Srđenović BU, Grujić NN, Šefer EI, Božin BN. Biochemical characterization of Helichrysum italicum (Roth) G. Don subsp. italicum (Asteraceae) from Montenegro: phytochemical screening, chemotaxonomy, and antioxidant properties. Chem Biodivers. 2015;12(3):419–31. doi: 10.1002/cbdv.201400174.

Oliva A, Garzoli S, Sabatino M, Tadić V, Costantini S, Ragno R, Božović M. Chemical composition and antimicrobial activity of essential oil of Helichrysum Italicum (Roth) G. Don Fil. (Asteraceae) from Montenegro. Nat Prod Res. 2020;34:445–8.

Fraternale D, Flamini G, Ascrizzi R. In Vitro Anticollagenase and Antielastase Activities of Essential Oil of Helichrysum italicum subsp. italicum (Roth) G. Don. J Med Food. 2019;22(10):1041–6.

Glumac M, Jažo Z, Paštar V, Golemac A, Čikeš Čulić V, Bektić S, et al. Chemical Profiling and Bioactivity Assessment of Helichrysum italicum (Roth) G. Don. Essential Oil: Exploring Pure Compounds and Synergistic Combinations. Molecules. 2023;28(14). doi: 10.3390/molecules28145299.

Prusinowska R, Śmigielski KB. Composition, biological properties and therapeutic effects of lavender (Lavandula angustifolia L). A review. Herba Pol. 2014;60(2):56–66. doi: 10.2478/hepo-2014-0010.

European Pharmacopoeia. 10th ed, Strasbourg: Council of Europe, 2019.

Assessment report on Lavandula angustifolia Miller, aetheroleum and Lavandula angustifolia Miller, flos [Internet]. European Medicines Agency; 2012 [cited 2024 May 21]. Available from: https://www.ema.europa.eu/en/documents/herbal-report/final-assessment-report-lavandula-angustifolia-miller-aetheroleum-and-lavandula-angustifolia-miller-flos_en.pdf.

Garzoli S, Laghezza Masci V, Franceschi S, Tiezzi A, Giacomello P, Ovidi E. Headspace/GC–MS Analysis and Investigation of Antibacterial, Antioxidant and Cytotoxic Activity of Essential Oils and Hydrolates from Rosmarinus officinalis L. and Lavandula angustifolia Miller. Foods. 2021;10(8). doi: 10.3390/foods10081768.

Community herbal monograph on Lavandula angustifolia Miller, flos [Internet]. European Medicines Agency; 2012 [cited 2024 May 21]. Available from: https://www.e-lactancia.org/media/papers/LavandaFlor-EMA2012.pdf.

Lis-Balchin M, Hart S. Studies on the mode of action of the essential oil of Lavender (Lavandula angustifolia P. Miller). Phytother Res. 1999;13(6):540–2.

Kotur-Stevuljevic J, Bogavac-Stanojevic N, Jelic-Ivanovic Z, Stefanovic A, Gojkovic T, Joksic J, et al. Oxidative stress and paraoxonase 1 status in acute ischemic stroke patients. Atherosclerosis. 2015;241(1):192–8. doi: 10.1016/j.atherosclerosis.2015.05.016.

Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38:1103-11.

Alamdari DH, Paletas K, Pegiou T, Sarigianni M, Befani C, Koliakos G. A novel assay for the evaluation of the prooxidant-antioxidant balance, before and after antioxidant vitamin administration in type II diabetes patients. Clin Biochem. 2007;40:248–54.

Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004;37:277–85.

Ellman GL. Tissue sulfhydril groups. Arch Biochem Biophys. 1959;82:70–7.

Mugao LG. Factors influencing yield, chemical composition and efficacy of essential oils. Int J Multidiscip Res Growth Eval. 2024;5:169–78. doi: 10.54660/.IJMRGE.2024.5.4.169-178.

Prusinowska R, Śmigielski K, Stobiecka A, Kunicka-Styczyńska A. Hydrolates from lavender (Lavandula angustifolia) – their chemical composition as well as aromatic, antimicrobial and antioxidant properties. Nat Prod Res. 2016;30(4):386–93. doi: 10.1080/14786419.2015.1016939.

Kunc N, Frlan A, Baričevič D, Kočevar Glavač N, Kokalj Ladan M. Essential Oil and Hydrosol Composition of Immortelle (Helichrysum italicum). Plants. 2022;11(19). doi: 10.3390/plants11192573.

Hydrosol Chemistry [Internet]. Circle H Institute; [cited 2024 May 21]. Available from: https://circlehinstitute.com/hydrosol-chemistry/.

Chen X, Shang S, Yan F, Jiang H, Zhao G, Tian S, et al. Antioxidant Activities of Essential Oils and Their Major Components in Scavenging Free Radicals, Inhibiting Lipid Oxidation and Reducing Cellular Oxidative Stress. Molecules. 2023;28(11). doi: 10.3390/molecules28114559.

Kozics K, Srancikova A, Sedlackova E, Horvathova E, Melusova M, Melus V, et al. Antioxidant potential of essential oil from Lavandula angustifolia in in vitro and ex vivo cultured liver cells. Neoplasma. 2017;64(4):485–93. doi: 10.4149/neo_2017_401.

Stanojević J, Stanojević Lj, Bulatović V, Zvezdanović J, Milenković A, Simonović N, et al. Chemical composition and antioxidant activity of immortelle (Helichrysum italicum) (Roth) G. Don) and yarrow (Achillea millefolium L.) essential oils. Adv Technol. 2022;11(1):93–103. doi: 10.5937/savteh2201093S.

But V, Bulboacă A, Rus V, Ilyés T, Gherman ML, Bolboacă SD. Anti-inflammatory and antioxidant efficacy of lavender oil in experimentally induced thrombosis. Thromb J. 2023;21(1). doi: 10.1186/s12959-023-00516-0.

Anthony KP, Deolu-Sobogun SA, Saleh MA. Comprehensive assessment of antioxidant activity of essential oils. J Food Sci. 2012;77(8). doi: 10.1111/j.1750-3841.2012.02795.x.

Mehri S, Meshki MA, Hosseinzadeh H. Linalool as a neuroprotective agent against acrylamide-induced neurotoxicity in Wistar rats. Drug Chem Toxicol. 2014;38(2):162–6. doi: 10.3109/01480545.2014.919585.

Deen JI, Zawad ANMS, Uddin M, Chowdhury MAH, Al Araby SQ, Rahman MA. Terpinen-4-ol, A volatile terpene molecule, extensively electrifies the biological systems against the oxidative stress-linked pathogenesis. Adv Redox Res. 2023;9:100082. doi: 10.1016/j.arres.2023.100082.

Mossa AT, Nawwar GA. Free radical scavenging and antiacetylcholinesterase activities of Origanum majorana L. essential oil. Hum Exp Toxicol. 2011;30(10):1501–13. doi: 10.1177/0960327110391686.

Kim HJ, Chen F, Wu CQ, Wang X, Chung HY, Jin ZY. Evaluation of antioxidant activity of Australian Tea Tree (Melaleuca alternifolia) oil and its components. J Agric Food Chem. 2004;52:2849–54.

Ozogul F, Çetinkaya A, El Abed N, Kuley E, Durmus M, Ozogul İ, et al. The effect of carvacrol, thymol, eugenol and α-terpineol in combination with vacuum packaging on quality indicators of anchovy fillets. Food Biosci. 2024;59:104008. doi: 10.1016/j.fbio.2024.104008.

Madhuri K, Naik PR. Ameliorative effect of borneol, a natural bicyclic monoterpene against hyperglycemia, hyperlipidemia and oxidative stress in streptozotocin-induced diabetic Wistar rats. Biomed Pharmacother. 2017;96:336–47.

Andreani S, Uehara A, Blagojević P, Radulović N, Muselli A, Baldovini N. Key odorants of industrially-produced Helichrysum italicum subsp. italicum essential oil. Ind Crops Prod. 2019;132:275–82.doi:10.1016j.indcrop.2019.02.008.

Ćavar Zeljković S, Šolić ME, Maksimović M. Volatiles of Helichrysum italicum (Roth) G. Don from Croatia. Nat Prod Res. 2015;29(19):1874–7. doi: 10.1080/14786419.2015.1009458.

Teixeira D, Lalot T, Brigodiot M, Maréchal E. β-Diketones as Key Compounds in Free-Radical Polymerization by Enzyme-Mediated Initiation. Macromolecules. 1999;32(1):70–2. doi: 10.1021/ma980872+.

Barzegar A, Moosavi-Movahedi AA. Intracellular ROS protection efficiency and free radical-scavenging activity of curcumin. PLoS One. 2011;6(10):e26012. doi: 10.1371/journal.pone.0026012.

Morales NP, Sirijaroonwong S, Yamanont P, Phisalaphong C. Electron Paramagnetic Resonance Study of the Free Radical Scavenging Capacity of Curcumin and Its Demethoxy and Hydrogenated Derivatives. Biol Pharm Bull. 2015;38(10):1478–83. doi: 10.1248/bpb.b15-00209.

Koroch AR, Juliani HR, Zygadlo JA. Bioactivity of essential oils and their components. In: Berger RG, editor. Flavours and Fragrances. Berlin: Springer; 2007; pp. 87‒115.

Juneja S, Rathore AS, Sharma K, Shetty D, Jain A. Antioxidant-Oxidant Index as a Biomarker in Oral Potentially Malignant Disorders and Oral Squamous Cell Carcinoma: A Biochemical Study. J Clin Diagn Res. 2017;11(3). doi: 10.7860/JCDR/2017/22909.9371.

Ilić M, Samardžić S, Kotur-Stevuljević J, Ušjak D, Milenković M, Kovačević N, Drobac M. Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents. Eur Rev Med Pharmacol Sci. 2021;25(20):6283–6294. doi: 10.26355/eurrev_202110_26998.

Kotur Stevuljević J, Savić J, Simić M, Ivanišević J. Redox homeostasis, oxidative stress and antioxidant system in health and disease: the possibility of modulation by antioxidants. Arh Farm. 2023;73(4):251–63. doi: 10.5937/arhfarm73-45369.