ANTIOXIDANT ACTIVITY OF HYDROLATE OBTAINED FROM THE AERIAL PART OF SWEET BASIL OCIMUM BASILICUM L.
Abstract
Ocimum basilicum is a well-known aromatic, medicinal, and culinary plant. The essential oils obtained through steam distillation from the leaves and flowering tops of sweet basil have antiseptic, antimicrobial, antioxidant, antiviral, and anti-inflammatory properties. As a product of hydrodestination hydrolates can be obtained. The main objective of the study was the spectrophotometric quantification of the content of total phenols, tannins, nontannins and flavonoids in the hydrolate obtained from aerial part of O. basilicum, the evaluation of antioxidant activity and the correlation analysis of certain phenolic compounds and antioxidant activity. Quantitative analysis of the concentration of total phenolic, flavonoid, tannin and nontannin in the hydrolate determined 151.91 ± 23.491mg CE/L , 23.34 ± 3.978 mg CE/L, 119.75 ± 8.09 mg CE/L and 0.86 ± 0.07 mg Ru/L, respectively. The hydrolate showed antioxidant potential in three assays for study: scavenged 2,2-diphenyl-1 picrylhydrazyl (DPPH) radicals with IC50 0.51 ± 1.07 %; total antioxidant potential, 392.15 ± 16.299 mmol of Fe2+/L and prevention of lipid peroxidation in a way that depends on concentration. In addition, correlation between phenolic compounds contents and antioxidant activity in hydrolated was noted. The demonstrated antioxidant properties of O. basilicum hydrolate may be crucial to its future as a potential natural antioxidant.
References
Aćimović M. Essential Oils: Inhalation aromatherapy - A comprehensive review. J Agron Technol Eng Manag 2021;4:547–57.
Baliyan S, Mukherjee R, Priyadarshini A, Vibhuti A, Gupta A, Pandey RP, et al. Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa. Molecules 2022;27(4):1326. [CrossRef] [PubMed]
Barbosa CO, de Morais SM, de Sousa HA, Martins VC, Neto JFC, Vieira IGP, et al. Chemical composition and antioxidant potential of essential oils from different Ocimum species (Basil). Braz J Dev Curitiba 2021;7:24422–42. [CrossRef]
Barros L, Ferreira MJ, Queiros B, Ferreira IC, Baptista P. Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chem 2007;103:413-9. [CrossRef]
Baydar H, Sangun MK, Erbas S, Kara N. Comparison of aroma compounds in distilled and extracted products of sage (Salvia officinalis L.). J Essent Oil-Bear Plants 2013;16:39-44. [CrossRef]
Boyraz N, Ozcan M. Antifungal effect of some spice hydrosols. Fitoterapia 2005;76:661-665. [CrossRef] [PubMed]
Camlica M, Yaldiz G, Ozen F. Effects of different basil hydrosol doses on the germination and shoot and root lengths of basil (Ocimum basilicum) and quinoa (Chenopodium quinoa) seeds. Indian J Pharm Educ Res 2017;51:S254-S257. [CrossRef] [PubMed]
Chaves N, Santiago A, Alías JC. Quantification of the antioxidant activity of plant extracts: analysis of sensitivity and hierarchization based on the method used. Antioxidants 2020; 9(1):76. [CrossRef] [PubMed]
D'Amato S, Serio A, López CC, Paparella A. Hydrosols: biological activity and potential as antimicrobials for food applications. Food Control 2018;86:126-37. [CrossRef]
Hamedi A, Moheimani SM, Sakhteman A, Etemadfard H, Moein M. An overview on indications and chemical composition of aromatic waters (hydrosols) as functional beverages in Persian nutrition culture and folk medicine for hyperlipidemia and cardiovascular conditions. J Evid Based Complementary Altern Med 2017; 22:544-61. [CrossRef] [PubMed]
Hamedi A, Pasdaran A, Zebarjad Z, Moein M. A survey on chemical constituents and indications of aromatic waters soft drinks (hydrosols) used in Persian nutrition culture and folk medicine for neurological disorders and mental health. J Evid Based Complementary Altern Med 2017;22(4):744-52. [CrossRef] [PubMed]
Hayyan M, Hashim MA, AlNashef IM. Superoxide ion: generation and chemical implications. Chem Rev 2016;116(5):3029–85. [CrossRef] [PubMed]
Jafri S, Khalid Z, Khan M, Jogezai N. Evaluation of phytochemical and antioxidant potential of various extracts from traditionally used medicinal plants of Pakistan. Open Chem 2022;20:1337-56. [CrossRef]
Jakubczyk K, Tuchowska A, Janda-Milczarek K. Plant hydrolates-antioxidant properties, chemical composition and potential applications. Biomed Pharmacother 2021;142:112033. [CrossRef] [PubMed]
Kačániová M, Galovičová L, Borotová P, Vukovic NL, Vukic M, Kunová S, et al. Assessment of Ocimum basilicum essential oil anti-insect activity and antimicrobial protection in fruit and vegetable quality. Plants 2022;11:1030. [CrossRef] [PubMed]
Kaya I, Yigit N, Benli M. Antimicrobial activity of various extracts of Ocimum basilicum L. and observation of the inhibition effect on bacterial cells by use of scanning electron microscopy. Afr J Tradit Complement Altern Med 2008;5:363–9. [CrossRef] [PubMed]
Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol 2011;48(4):412-22. [CrossRef] [PubMed]
Koleva II, Van Beek TA, Linssen JPH, De Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal 2002;13(1):8–17. [CrossRef] [PubMed]
Kumar Sinha A, Singh TP. A new chemotype of Ocimum basilicum var. purpurascens Benth. from Madhubani district of Bihar, India and phytochemical investigations of their colchiploids. Int J Fauna Biol Stud 2021;8:14–18. [CrossRef]
Kunicka SA, Śmigielski K, Prusinowska R, Rajkowska K, Kuśmider B, Sikora M. Preservative activity of lavender hydrosols in moisturizing body gels. Lett Appl Microbiol 2015;60:27-32. [CrossRef] [PubMed]
Laguerre M, Lecomte J, Villeneuve P. Evaluation of the ability of antioxidants to counteract lipid oxidation: existing methods, new trends and challenges. Prog Lipid Res 2007;46:244-82. [CrossRef] [PubMed]
Lamaison JL, Carnat A. Teneurs en principaux flavonoids des fleurs de Crataegeus monogyna Jacq et de Crataegeus laevigata (Poiret D. C.) en fonction de la vegetation. Pharm Acta Helv 1990;65:315-20.
Makkar HPS, Hagerman A, Mueller-Harvey I. Quantification on tannins in tree foliage-a laboratory manual. FAO/IAEA:Vienna. 2000. https://www.iaea.org/sites/default/files/21/06/nafa-aph-manual-pubd31022manual-tannin.pdf
Maksimović Z, Malenčić Đ, Kovačević N. Polyphenol contents and antioxidant activity of Maydis stigma extracts. Bioresour Technol 2005;96:873–7. [CrossRef] [PubMed]
Mkaddem Mounira G, Ahlem Z, Abdallah Mariem B, Romdhane M, Okla MK, Al-Hashimi A, et al. Essential oil composition and antioxidant and antifungal activities of two varieties of Ocimum basilicum L. (Lamiaceae) at two phenological stages. Agronomy 2022;12:825. [CrossRef]
Monsef EH, Amanzade Y, Alhani Z, Hajimehdipour H, Faramarzi MA. GC/MS analysis of Citrus aurantium L. hydrolate and its comparison with the commercial samples. Iran J Pharm Res 2004;3:177-179. [CrossRef]
Munteanu IG, Apetrei C. Analytical methods used in determining antioxidant activity: A Review. Int J Mol Sci 2021;22(7):3380. [CrossRef] [PubMed]
Pavlović DR, Tasić-Kostov M, Marčetić M, Lakušić B, Kitić D, Savić S, et al. Evaluation of in vivo effects on surfactant-irritated human skin, antioxidant properties and phenolic composition of five Ericaceae species extracts. RSC Adv 2013;90:255-264.
Pavlović DR, Veljković M, Stojanović NM, Gočmanac-Ignjatović M, Mihailov-Krstev T, Branković S, et al. Influence of different wild-garlic (Allium ursinum) extracts on the. J Pharm Pharmacol 2017;69:1208-18. [CrossRef] [PubMed]
Pellegrini N, Serafini M, Colombi B, Del Rio D, Salvatore S, Bianchi M, et al. Total antioxidant capacity of plant foods, beveragesand oils consumed in Italy assessed by three different in vitro assays. J Nutr 2003;133:2812-2819. [CrossRef] [PubMed]
Peschel W, Sánchez-Rabaneda F, Diekmann W, Plescher A, Gartzía I, Jimenez D, et al. An industrial approach in the search of natural antioxidants from vegetable and fruit wastes. Food Chem 2006;97:137–50. [CrossRef]
Reddy GM, Rao V, Sarma D, Reddy TK, Subramanyam P, Naidu MD. Evaluation of antioxidant activity index (AAI) by the 2, 2-diphenyl-1-picryl hydrazyl method of 40 medicinal plants. J Med Plants Res 2012;6:4082-6. [CrossRef]
Rice-Evans C, Miller N, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci 1997;2:152–9. [CrossRef]
Saw CLL, Guo Y, Yang AY, Paredes-Gonzalez X, Ramirez C, Pung D, et al. The berry constituents quercetin, kaempferol, and pterostilbene synergistically attenuate reactive oxygen species: Involvement of the Nrf2-ARE signaling pathway. Food Chem Toxicol 2014;72:303–11. [CrossRef] [PubMed]
Smigielski KB, Prusinowska R, Bemska JE. Comparison of the chemical composition of essential oils and hydrolates from basil (Ocimum basilicum L.). J Essent Oil-Bear Plants 2016;19:492-8. [CrossRef]
Šovljanski O, Saveljić A, Aćimović M, Šeregelj V, Pezo L, Tomić A, et al. Biological profiling of essential oils and hydrolates of Ocimum basilicum var. genovese and var. minimum originated from Serbia. Processes 2022;10:1893. [CrossRef]
Tavares CS, Gameiro JA, Roseiro LB, Figueiredo AC. Hydrolates: A review on their volatiles composition, biological properties and potential uses. Phytochem Rev 2022;21:1661-737. [CrossRef]
